微囊化沙门菌SP4噬菌体的制备及其缓释特性分析

通过采用阳离子醚化淀粉/海藻酸钠/黄原胶/纳米TiO₂/壳寡糖为原料制备微囊化SP4噬菌体,为控制沙门菌感染提供生物制剂。选用沙门菌SP4噬菌体,采用液滴法来制备微囊化SP4噬菌体,并对其效价、阳离子醚化淀粉浓度、海藻酸钠浓度、黄原胶浓度、壳寡糖浓度、pH稳定性、热稳定性、模拟胃液稳定性、模拟肠液释放行为以及保存稳定性进行分析。结果显示,当阳离子醚化淀粉浓度为2.4%、海藻酸钠浓度为2%、黄原胶浓度为1%、壳寡糖浓度为0.6%时,微囊化SP4噬菌体微球外形规则,包封率为97.5%,在pH5.0~8.0、温度10~30℃、模拟胃液pH2.0、0~30 min和pH3.0、0~150 min噬菌体保持较高的生物学活性,模拟肠液中4 h,噬菌体完全释放,4℃环境下保存6周噬菌体效价无明显降低。综上表明,微囊化沙门菌SP4噬菌体显著提高了噬菌体的稳定性和缓释性能,其耐酸特性利于抵御胃酸的分解,具有应用于生物防治的潜力,为进一步采用多因素试验制备微囊化沙门菌SP4噬菌体奠定了基础。     

氮磷肥对季节性栽培紫花苜蓿生长及再生的影响

为探讨江淮地区紫花苜蓿季节性栽培体系中氮磷肥供应对干物质产量及再生的影响,试验设置4个磷（P₂O₅）水平处理（0、50、100、150 kg·hm^-2）及4个氮（N）水平处理（0、60、120、180 kg·hm^-2）,研究了干物质产量及产量构成因子、地上部氮磷含量及累积量、再生6和12 d的生长量等指标对氮磷肥投入的响应。结果表明：1）施用氮肥及磷肥均显著促进了紫花苜蓿的生长。在低磷供应条件下,干物质产量随供氮量的增加而增加;在高磷条件下,适宜生长的最优施氮量为120 kg·hm^-2。对不同施氮处理而言,饲草干物质产量均随施磷量的增加显著增加。2）干物质产量与地上部氮含量、地上部氮累积量、地上部磷累积量间存在显著正相关关系。3）氮磷肥施用可以促进植株残茬再生,0、50、100、150 kg·hm^-2磷处理下适宜残茬再生所需的施氮量分别为180、120、120、60 kg·hm^-2。刈割6 d后残茬的再生芽芽长及叶面积、刈割12 d后叶面积均与再生生物量间存在显著正相关关系。可见,江淮地区紫花苜蓿季节性栽培体系中施用磷肥可在一定程度上减少氮肥的用量。当磷肥施用量分别为0、50、100、150 kg·hm^-2时,适宜生长及再生的氮肥推荐用量分别为180、120、120、60 kg·hm^-2。江淮地区紫花苜蓿季节性栽培体系中推荐年施磷量及施氮量分别为100及120 kg·hm^-2,研究结果可为紫花苜蓿季节性栽培技术中的肥料管理提供理论依据。     

控释氮肥对棉花产量的影响及光合特性的研究

在大田条件下,以普通尿素常规施肥量(N 150 kg.hm-2)为对照,研究了控释氮肥不同用量和不同施用方法对棉花光合特性和产量的影响,结果表明:控释氮肥处理可提高中后期棉叶叶肉细胞PSⅡ实际光化学效率(ФPSⅡ)和PSⅡ潜在光化学活性(Fv/Fo),改善叶肉细胞的光合能力,提高叶片光合效率,从而提高群体光合效率,增加单株结铃数和铃重。和对照比,控释氮肥各处理子棉产量和肥效表现:(1)等氮量(施N 150 kg.hm-2)基施处理极显著增产10.3%,肥效提高10.2%;(2)等氮量(施N 150 kg.hm-2)1∶1基追施处理极显著增产22.5%,肥效提高22.4%;(3)施氮120 kg.hm-21∶1基追施处理极显著增产12.3%,肥效提高40.3%;(4)施N 120 kg.hm-2基施处理增产3.2%,肥效提高29.4%。     

A geographical approach to identify phosphorus-efficient genotypes among landraces and wild ancestors of common bean

Cultivars of common bean (Phaseolus vulgaris L.) capable of yielding well at low levels of native or added phosphorus (P) are highly desirable in many tropical production systems. The objective of the present study was to identify geographical regions which might be sources of such genotypes. A total of 364 landraces, cultivars and wild genotypes, drawn from a broad geographic range, were divided on the basis of growth habit into four field trials, each comprising two levels of P, stressed and unstressed, on an infertile Andosol in Popayan, Colombia. The regression relationship between grain yield per plant in the presence and in the absence of stress was determined, and each genotype's deviation from this relationship was used as a measure of P-efficiency. There was highly significant variation in efficiency among genotypes in all growth habits, and in climbing beans there were consistent regional differences, superior genotypes being identified with greater frequency among those from Bolivia, West Mexico and South Mexico-West Guatemala. The latter region was promising for prostrate bush genotypes also. Wild beans in general performed relatively poorly; it appears that P-efficiency traits in P. vulgaris have been acquired during or after domestication. These results confirm that genetic differences in P-efficiency exist among common bean genotypes and suggest that these are related to geographic origin. Furthermore, the use of a representative sample of germplasm can help to identify segments of the gene bank that are especially promising as sources of desirable traits. This revised version was published online in July 2006 with corrections to the Cover Date.     

低磷胁迫下水稻不同品种根系有机酸分泌的差异

以4个对低磷反应不同的典型水稻品种（系）为材料，对其在低磷胁迫下根系有机酸分泌这一根系生理特性进行了比较研究。结果表明，低磷胁迫能促使水稻根系分泌更多的有机酸，这在耐低磷品种中表现尤为明显。     

Non‐phytate phosphorus requirements and efficacy of a genetically engineered yeast phytase in male lingnan yellow broilers from 1 to 21 days of age

This experiment was conducted to investigate the requirement of non‐phytate phosphorus (nPP) and efficacy of a genetically engineered yeast phytase in performance and tibia characteristics by male Lingnan Yellow broilers from 1 to 21 days of age. A total of 2640 1‐day‐old male chicks were randomly allotted to one of 11 dietary treatments, which consisted of six replicate floor pens with 40 birds per pen. All treatments had the same levels of all nutrients except for phosphorus and phytase. The control group (treatment 1) was fed the basal diet without dicalcium phosphate or phytase supplementation. Dietary concentrations of nPP were 0.11%, 0.19%, 0.27%, 0.35%, 0.43%, 0.51% and 0.59% respectively for treatments 1, 2, 3, 4, 5, 6 and 7, through addition of dicalcium phosphate (chemistry grade) to the basal diet. Diets 8–11 were supplemented with a genetically engineered yeast phytase 250, 500, 750 U/kg and a commercial phytase product 500 U/kg in basal diet respectively. The results showed that 0.46% and 0.51% nPP were required for maximum body‐weight gain and optimum tibia development indicators respectively. However, 0.59% nPP had a negative effect on bird growth. The equivalency value of the genetically engineered yeast phytase was estimated to be 874 U/kg to liberate 0.1% nPP.     

施用豆科绿肥对砖红壤有效磷含量的影响

土壤缺磷是热带和亚热带地区作物生产的主要限制因子之一。本研究分析了14份热带、亚热带豆科绿肥施入土壤后对砖红壤有效磷含量影响的时间动态变化。结果表明,施用绿肥后不同时段能不同程度地提高土壤有效磷的含量,且施用绿肥后的土壤有效磷含量显著高于不施用绿肥土壤,其中木蓝（Magnolia lotungensis）对砖红壤有效磷含量增量变幅高达339%。此外,施用不同的绿肥对土壤有效磷含量的影响不同,但对土壤有效磷含量影响的基本规律一致。施用绿肥时砖红壤有效磷含量提高较大的最佳埋田时间为1个月。     

低蛋白低磷加酶日粮对生长猪生产性能及减排效果的影响

选择30头24.4kg左右的健康杜×长×大杂交猪,将其随机分成2组,每组3个重复,每个重复5头猪,分别饲喂两种日粮：对照组日粮（18.4%CP＋0.37%有效磷,高蛋白高磷组日粮）和试验组日粮（16.0%CP＋0.28%有效磷＋100g/t植酸酶,低蛋白低磷加酶组日粮）,研究低蛋白低磷加酶日粮对生长猪生产性能及减排效果的影响。结果表明,低蛋白低磷加酶日粮对生长猪生产性能没有显著影响,但是可显著提高氮、磷的利用率,从而减少氮、磷排放。     

过量磷对菜心植株生长和产量及品质的影响

采用营养液水培法,研究过量磷对菜心(Brassica parachinensis)植株生长、产量和品质的影响.结果表明:与正常磷浓度处理(1 mmol/L H2PO4-)比较,3～7mmol/L H2P04-处理植株生物量和产量均显著降低,5和7 mmol/L H2PO4-处理植株根冠比显著增加;随着磷浓度的增加,菜薹表皮花色苷含量和菜薹可溶性糖含量逐渐增加,菜薹硝酸盐含量逐渐降低,三者与营养液磷浓度均呈显著线性关系.     

E_1级麻屑板用脲醛树脂胶的研究

根据亚麻屑的胶接特性,从脲醛胶的固体含量、粘度、游离甲醛、耐水性、固化时间、施工胶液特性及经济效益等方面系统地研究了适合低甲醛释放量麻屑板生产用脲醛树脂胶的物理力学及化学性能。研究结果表明,使用改进的脲醛树脂胶(命名为DN—9号和DN—11号低毒腮醛胶)可以压制出甲醛释放量小于10mg/100g的E1级麻屑板,其物理力学性能达到GB 4896—4905—35标准